私たちが作製したタンパク質の結晶とその立体構造の一覧です。
(宮崎が学生時代に作った、懐かし~~結晶も含まれます)
ネット上では偏光板を通して撮影した綺麗な色の結晶の写真が多く見られますが、実際は無色透明が多いです。結晶の写真の縮尺はバラバラですが、一辺がだいたい数十~数百 μmの結晶が多いです。
GH97 (EC 3.2.1.70) Glucodextranase from Flavobacterium johnsoniae PDB ID 8WG0, 8WG1, 8WG2 Nakamura, Kurata et al., FEBS J. 2024 |
GH31_19 (EC 3.2.1.22) α-1,4-Galactosidase from Bacteroides salyersiae PDB ID 8J53 Ikegaya et al., FEBS J. 2023 |
GH31_19 (EC 3.2.1.22) α-1,4-Galactosidase from Flavihumibacter petaseus PDB ID 8J50, 8J51, 8J52 Ikegaya et al., FEBS J. 2023 |
GH66 (EC 3.2.1.11) Dextranase from Flavobacterium johnsoniae cocrystallized with isomaltotriose (P212121 space group) PDB ID 8IUB Nakamura et al., J. Biol. Chem. 2023 |
GH66 (EC 3.2.1.11) Dextranase from Flavobacterium johnsoniae (C2 space group) PDB ID 8IU8, 8IU9, 8IUA Nakamura et al., J. Biol. Chem. 2023 |
CBM94 C-terminal domain of N-acetylglucosaminyltransferase IV (GnT-IV, GT54) ortholog from Bombyx mori PDB ID 7XTM, 7XTN Oka, Mori et al., Glycobiology 2022 |
CBM94 C-terminal domain of N-acetylglucosaminyltransferase IV a (GnT-IVa, GT54) from Homo sapiens PDB ID 7XTL Oka, Mori et al., Glycobiology 2022 |
GH31 (EC 3.2.1.84) α-1,3-Glucosidase from Lactococcus lactis (P21 space group) PDB ID 7WJ9 (cryo-EM → 7WLG, EMD-32571) Ikegaya et al., J. Biol. Chem. 2022 |
GH31 (EC 3.2.1.84) α-1,3-Glucosidase from Lactococcus lactis (P6322 space group) PDB ID 7WJA, 7WJB, 7WJC, 7WJD, 7WJE, 7WJF Ikegaya et al., J. Biol. Chem. 2022 |
GH65 (EC 3.2.1.216) Kojibiose hydrolase (α-1,2-Glucosidase) from Flavobacterium johnsoniae PDB ID 7FE3, 7FE4, 8IUC Nakamura et al., J. Biol. Chem. 2021 Nakamura et al., J. Biol. Chem. 2023 |
GH92 (EC 3.2.1.113) α-1,2-Mannosidase from Enterococcus faecalis PDB ID 7FE1, 7FE2 Alonso-Gil et al., Chem. Eur. J. 2022 |
GH32 (EC 3.2.1.26) β-Fructofuranosidase from Bombyx mori PDB ID 7BWB, 7BWC Miyazaki et al., Insect Biochem. Mol. Biol. 2020 |
GH13_17 (EC 3.2.1.48) Sucrose hydrolase from Bombyx mori PDB ID 6LGA, 6LGB, 6LGC, 6LGD, 6LGE, 6LGF, 6LGG, 6LGH, 6LGI Miyazaki and Park, J. Biol. Chem. 2020 |
GH31 (EC 3.2.1.217) α-N-Acetylgalactosaminidase from Enterococcus faecalis PDB ID 6M76, 6M77, 7F7Q, 7F7R Miyazaki and Park, FEBS Lett. 2020 Miyazaki et al., Biochimie 2022 |
GH63 (EC 3.2.1.170) Mannosylglycerate hydrolase from Thermus thermophilus PDB ID 4WVA, 4WVB, 4WVC Miyazaki et al., J. Struct. Biol. 2015 |
GH31 (EC 3.2.1.22) α-Galactosidase from Pseudopedobacter saltans PDB ID 4XPO, 4XPP, 4XPQ, 4XPR, 4XPS Miyazaki et al., Biochem. J. 2015 |
GH63 (EC 3.2.1.-) α-Glucoside hydrolase (mutant) from Escherichia coli PDB ID 3W7W, 3W7X, 5CA3, 5GW7 Miyazaki et al., FEBS J. 2013 Miyazaki et al., J. Struct. Biol. 2016 |
GH131 CcGH131A from Coprinopsis cinerea PDB ID 3W9A Miyazaki et al., FEBS Lett. 2013 |
GH49 (EC 3.2.1.57) Isopullulanase (mutant) from Aspergillus brasiliensis PDB ID 3WWG Miyazaki et al., J. Biochem. 2015 |
GH6 (EC 3.2.1.91) Cellobiohydrolase CcCel6A from Coprinopsis cinerea PDB ID 3VOG, 3VOH, 3VOI, 3VOJ Tamura et al., FEBS J. 2012 |