結晶構造ギャラリー

私たちが作製したタンパク質の結晶とその立体構造の一覧です。
(宮崎が学生時代に作った、懐かし~~結晶も含まれます)
ネット上では偏光板を通して撮影した綺麗な色の結晶の写真が多く見られますが、実際は無色透明が多いです。結晶の写真の縮尺はバラバラですが、一辺がだいたい数十~数百 μmの結晶が多いです。


An enzyme
unpublished

GH31 (EC 3.2.1.84)
α-1,3-Glucosidase from Lactococcus lactis (P21 space group)
PDB ID 7WJ9 (cryo-EM → 7WLG, EMD-32571)
Ikegaya et al., J. Biol. Chem. 2022

GH31 (EC 3.2.1.84)
α-1,3-Glucosidase from Lactococcus lactis (P6322 space group)
PDB ID 7WJA, 7WJB, 7WJC, 7WJD, 7WJE, 7WJF
Ikegaya et al., J. Biol. Chem. 2022

GH65 (EC 3.2.1.216)
Kojibiose hydrolase (α-1,2-Glucosidase) from Flavobacterium johnsoniae
PDB ID 7FE3, 7FE4
Nakamura et al., J. Biol. Chem. 2021

GH92 (EC 3.2.1.113)
α-1,2-Mannosidase from Enterococcus faecalis
PDB ID 7FE1, 7FE2
Alonso-Gil et al., Chem. Eur. J. 2022

GH32 (EC 3.2.1.26)
β-Fructofuranosidase from Bombyx mori
PDB ID 7BWB, 7BWC
Miyazaki et al., Insect Biochem. Mol. Biol. 2020

GH13_17 (EC 3.2.1.48)
Sucrose hydrolase from Bombyx mori
PDB ID 6LGA, 6LGB, 6LGC, 6LGD, 6LGE, 6LGF, 6LGG, 6LGH, 6LGI
Miyazaki and Park, J. Biol. Chem. 2020

GH31 (EC 3.2.1.217)
α-N-Acetylgalactosaminidase from Enterococcus faecalis
PDB ID 6M76, 6M77, 7F7Q, 7F7R
Miyazaki and Park, FEBS Lett. 2020
Miyazaki et al., Biochimie 2022

GH63 (EC 3.2.1.170)
Mannosylglycerate hydrolase from Thermus thermophilus
PDB ID 4WVA, 4WVB, 4WVC
Miyazaki et al., J. Struct. Biol. 2015

GH31 (EC 3.2.1.22)
α-Galactosidase from Pseudopedobacter saltans
PDB ID 4XPO, 4XPP, 4XPQ, 4XPR, 4XPS
Miyazaki et al., Biochem. J. 2015

GH63 (EC 3.2.1.-)
α-Glucosidase (mutant) from Escherichia coli
PDB ID 3W7W, 3W7X, 5CA3, 5GW7
Miyazaki et al., FEBS J. 2013
Miyazaki et al., J. Struct. Biol. 2016

GH131
CcGH131A from Coprinopsis cinerea
PDB ID 3W9A
Miyazaki et al., FEBS Lett. 2013

GH49 (EC 3.2.1.57)
Isopullulanase (mutant) from Aspergillus brasiliensis
PDB ID 3WWG
Miyazaki et al., J. Biochem. 2015

GH6 (EC 3.2.1.91)
Cellobiohydrolase CcCel6A from Coprinopsis cinerea
PDB ID 3VOG, 3VOH, 3VOI, 3VOJ
Tamura et al., FEBS J. 2012