論文・著書

原著論文

*責任著者(corresponding author)、太字は宮崎または指導学生

2022年

38. Marina Ikegaya, Toshio Moriya, Naruhiko Adachi, Masato Kawasaki, Enoch Y. Park, Takatsugu Miyazaki*  (2022) Structural basis of the strict specificity of a bacterial GH31 α-1,3-glucosidase for nigerooligosaccharides. Journal of Biological Chemistry 298, 101827. https://doi.org/10.1016/j.jbc.2022.101827

37. Santiago Alonso-Gil*, Kamil Parkan, Jakub Kaminský, Radek Pohl, Takatsugu Miyazaki* (2022) Unlocking the hydrolytic mechanism of GH92 α-1,2-mannosidases: computation inspires using C-glycosides as Michaelis complex mimics. Chemistry – A European Journal 28, e202200148. https://doi.org/10.1002/chem.202200148

36. Takatsugu Miyazaki*, Marina Ikegaya, Santiago Alonso-Gil* (2022) Structural and mechanistic insights into the substrate specificity and hydrolysis of GH31 α-N-acetylgalactosaminidase. Biochimie 195, 90–99. https://doi.org/10.1016/j.biochi.2021.11.007

2021年

35. Shuntaro Nakamura, Takanori Nihira, Rikuya Kurata, Hiroyuki Nakai, Kazumi Funane, Enoch Y. Park, Takatsugu Miyazaki* (2021) Structure of a bacterial α-1,2-glucosidase defines mechanisms of hydrolysis and substrate specificity in GH65 family hydrolases. Journal of Biological Chemistry 297, 101366. https://doi.org/10.1016/j.jbc.2021.101366

34. Marina Ikegaya, Takatsugu Miyazaki*, Enoch Y. Park (2021) Biochemical characterization of Bombyx mori α-N-acetylgalactosaminidase belonging to the glycoside hydrolase family 31. Insect Molecular Biology 30, 367–378. https://doi.org/10.1111/imb.12701

2020年

33. Takatsugu Miyazaki*, Nozomi Oba, Enoch Y. Park (2020) Structural insight into the substrate specificity of Bombyx mori β-fructofuranosidase belonging to the glycoside hydrolase family 32. Insect Biochemistry and Molecular Biology 127, 103494. https://doi.org/10.1016/j.ibmb.2020.103494

32. Shuntaro Nakamura, Takatsugu Miyazaki*, Enoch Y. Park (2020) α-L-Fucosidase from Bombyx mori has broad substrate specificity and hydrolyzes core fucosylated N-glycans. Insect Biochemistry and Molecular Biology 124, 103427. https://doi.org/10.1016/j.ibmb.2020.103427

31. Takatsugu Miyazaki*, Enoch Y. Park (2020) Crystal structure of the Enterococcus faecalis α-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31. FEBS Letters 594, 2282–2293. https://doi.org/10.1002/1873-3468.13804

30. Takatsugu Miyazaki*, Enoch Y. Park (2020) Structure–function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17 exo-α-glucosidases. Journal of Biological Chemistry 295, 8784–8797. https://doi.org/10.1074/jbc.RA120.013595

2019年

29. Takatsugu Miyazaki, Ryunosuke Miyashita, Shuntaro Nakamura, Marina Ikegaya, Tatsuya Kato, Enoch Y. Park* (2019) Biochemical characterization and mutational analysis of silkworm Bombyx mori β-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1,4-galactosyltransferase family enzymes. Insect Biochemistry and Molecular Biology 115, 103254. https://doi.org/10.1016/j.ibmb.2019.103254

28. Daiki Mizushima, Takatsugu Miyazaki (co-first author), Yuh Shiwa, Keitarou Kimura, Shiho Suzuki, Nobuyuki Fujita, Hirofumi Yoshikawa, Atsuo Kimura, Shinichi Kitamura, Hiroshi Hara, Kazumi Funane* (2019) A novel intracellular dextranase derived from Paenibacillus sp. 598K with an ability to degrade cycloisomaltooligosaccharides. Applied Microbiology and Biotechnology 103, 6581–6592. https://doi.org/10.1007/s00253-019-09965-y

27. Vipin Kumar Deo, Yu Inagaki, Elsa Herdiana Murhandarwati, Widya Asmara, Takatsugu Miyazaki, Tatsuya Kato, Enoch Y. Park* (2019) Sero-diagnostic potential of Plasmodium falciparum recombinant Merozoite Surface Protein (MSP)-3 expressed in silkworm. Parasitology International 72, 101938. https://doi.org/10.1016/j.parint.2019.101938

26. Keiji Fushimi, Takatsugu Miyazaki, Yuto Kuwasaki, Takahiro Nakajima, Tatsuro Yamamoto, Kazushi Suzuki, Yoshibumi Ueda, Keita Miyake, Yuka Takeda, Jae-Hoon Choi, Hirokazu Kawagishi, Enoch Y. Park, Masahiko Ikeuchi, Moritoshi Sato, Rei Narikawa* (2019) Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Proceedings of the National Academy of Sciences of the United States of America 116, 8301–8309. https://doi.org/10.1073/pnas.1818836116

25. Takatsugu Miyazaki, Ryunosuke Miyashita, Sota Mori, Tatsuya Kato, Enoch Y. Park* (2019) Expression and characterization of silkworm Bombyx mori β-1,2-N-acetylglucosaminyltransferase II, a key enzyme for complex-type N-glycan biosynthesis. Journal of Bioscience and Bioengineering 127, 273–280. https://doi.org/10.1016/j.jbiosc.2018.08.014

2018年

24. Tatsuya Kato, Moeko Hasegawa, Takeshi Yamamoto, Takatsugu Miyazaki, Ryosuke Suzuki, Takaji Wakita, Tetsuro Suzuki, Enoch Y. Park* (2018) Expression of a functional intrabody against hepatitis C virus core protein in Escherichia coli and silkworm pupae. Protein Expression and Purification 150, 61–66. https://doi.org/10.1016/j.pep.2018.05.009

23. Ryota Yagi, Takatsugu Miyazaki, Takanori Oyoshi* (2018) G-quadruplex binding ability of TLS/FUS depends on the β-spiral structure of the RGG domain. Nucleic Acids Research 46, 5894–5901. https://doi.org/10.1093/nar/gky391

22. Kazumi Funane, Yukinori Tanaka, Takeshi Hosaka, Kiriko Murakami, Takatsugu Miyazaki, Yuh Shiwa, Shigehachi Gibu, Takeshi Inaoka, Ken Kasahara, Nobuyuki Fujita, Hirofumi Yoshikawa, Yoshikazu Hiraga, Kozo Ochi* (2018) Combined drug-resistance mutations substantially enhance enzyme production in Paenibacillus agaridevorans. Journal of Bacteriology 200, e00188-18. https://doi.org/10.1128/JB.00188-18

21. Takatsugu Miyazaki, Tatsuya Kato, Enoch Y. Park* (2018) Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm–BmNPV bacmid system. Journal of Bioscience and Bioengineering 126, 15–22. https://doi.org/10.1016/j.jbiosc.2018.01.011

2017年

20. Takatsugu Miyazaki, Masaaki Ishizaki, Hideo Dohra, Sungjo Park, Andre Terzic, Tatsuya Kato, Tetsuya Kohsaka, Enoch Y. Park* (2017) Insulin-like peptide 3 expressed in the silkworm possesses intrinsic disulfide bonds and full biological activity. Scientific Reports 7, 17339. https://doi.org/10.1038/s41598-017-17707-1

19. Tatsuya Kato, Natsumi Kako, Kotaro Kikuta, Takatsugu Miyazaki, Sachiko Kondo, Hirokazu Yagi, Koichi Kato, Enoch Y. Park* (2017) N-Glycan modification of a recombinant protein via coexpression of human glycosyltransferases in silkworm pupae. Scientific Reports 7, 1409. https://doi.org/10.1038/s41598-017-01630-6

18. Hitomi Ichinose, Ryuichiro Suzuki, Takatsugu Miyazaki, Keitarou Kimura, Mitsuru Momma, Nobuhiro Suzuki, Zui Fujimoto, Atsuo Kimura, Kazumi Funane* (2017) Paenibacillus sp. 598K 6-α-glucosyltransferase is essential for cycloisomaltooligosaccharide synthesis from α-(1→4)-glucan. Applied Microbiology and Biotechnology 101, 4115–4128. https://doi.org/10.1007/s00253-017-8174-z

17. Takashi Tonozuka*, Yutaro Tanaka, Shunsaku Okuyama, Takatsugu Miyazaki, Atsushi Nishikawa, Makoto Yoshida (2017) Structure of the catalytic domain of α-L-arabinofuranosidase from Coprinopsis cinerea, CcAbf62A, provides insights into structure–function relationships in glycoside hydrolase family 62. Applied Biochemistry and Biotechnology 181, 511–525. https://doi.org/10.1007/s12010-016-2227-0

2016年(静大着任年)

16. Takatsugu Miyazaki, Atsushi Nishikawa, Takashi Tonozuka* (2016) Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase. Journal of Structural Biology 196, 479–486. https://doi.org/10.1016/j.jsb.2016.09.015

15. Yoshifumi Gozu, Yuichi Ishizaki, Yuhei Hosoyama, Takatsugu Miyazaki, Atsushi Nishikawa, Takashi Tonozuka* (2016) A glycoside hydrolase family 31 dextranase with high transglucosylation activity from Flavobacterium johnsoniae. Bioscience, Biotechnology, and Biochemistry 80, 1562–1567. https://doi.org/10.1080/09168451.2016.1182852

14. Marina Mori, Megumi Ichikawa, Yumiko Kiguchi, Takatsugu Miyazaki, Makoto Hattori, Atsushi Nishikawa, Takashi Tonozuka* (2016) A surface loop in the N-terminal domain of Pedobacter heparinus heparin lyase II is important for activity. Journal of Applied Glycoscience 63, 7–11. https://doi.org/10.5458/jag.jag.JAG-2015_019

2015年

13. Yuka Okazawa, Takatsugu Miyazaki, Gaku Yokoi, Yuichi Ishizaki, Atsushi Nishikawa, Takashi Tonozuka* (2015) Crystal structure and mutational analysis of isomalto-dextranase, a member of glycoside hydrolase family 27. Journal of Biological Chemistry 290, 26339–26349. https://doi.org/10.1074/jbc.M115.680942

12. Takatsugu Miyazaki, Yuichi Ishizaki, Megumi Ichikawa, Atsushi Nishikawa, Takashi Tonozuka* (2015) Structural and biochemical characterization of novel bacterial α-galactosidases belonging to glycoside hydrolase family 31. Biochemical Journal 469, 145–158. https://doi.org/10.1042/BJ20150261

11. Takatsugu Miyazaki, Hiroyuki Yashiro, Atsushi Nishikawa, Takashi Tonozuka* (2015) The side chain of a glycosylated asparagine residue is important for the stability of isopullulanase. Journal of Biochemistry 157, 225–234. https://doi.org/10.1093/jb/mvu065

10. Emiko Takagi, Yuji Hatada*, Masatake Akita, Yukari Ohta, Gaku Yokoi, Takatsugu Miyazaki, Atsushi Nishikawa, Takashi Tonozuka* (2015) Crystal structure of the catalytic domain of a GH16 β-agarase from a deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94. Bioscience, Biotechnology, and Biochemistry 79, 625–632. https://doi.org/10.1080/09168451.2014.988680

9. Takatsugu Miyazaki, Megumi Ichikawa, Hitoshi Iino, Atsushi Nishikawa, Takashi Tonozuka* (2015) Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8. Journal of Structural Biology 190, 21–30. https://doi.org/10.1016/j.jsb.2015.02.006

2014年

8. Yukari Ohta, Yuji Hatada*, Yuko Hidaka, Yasuhiro Shimane, Keiko Usui, Tetsuya Ito, Koki Fujita, Gaku Yokoi, Marina Mori, Shona Sato, Takatsugu Miyazaki, Atsushi Nishikawa, Takashi Tonozuka* (2014) Enhancing thermostability and the structural characterization of Microbacterium saccharophilum K-1 β-fructofuranosidase. Applied Microbiology and Biotechnology 98, 6667–6677. https://doi.org/10.1007/s00253-014-5645-3

2013年

7. Takatsugu Miyazaki, Megumi Ichikawa, Gaku Yokoi, Motomitsu Kitaoka, Haruhide Mori, Yoshikazu Kitano, Atsushi Nishikawa, Takashi Tonozuka* (2013) Structure of a bacterial glycoside hydrolase family 63 enzyme in complex with its glycosynthase product, and insights into the substrate specificity. FEBS Journal 280, 4560–4571. https://doi.org/10.1111/febs.12424

6. Takatsugu Miyazaki, Makoto Yoshida, Mizuki Tamura, Yutaro Tanaka, Kiwamu Umezawa, Atsushi Nishikawa, Takashi Tonozuka* (2013) Crystal structure of the N-terminal domain of a glycoside hydrolase family 131 protein from Coprinopsis cinerea. FEBS Letters 587, 2193–2198. https://doi.org/10.1016/j.febslet.2013.05.041

2012年

5. Takashi Tonozuka*, Akiko Tamaki, Gaku Yokoi, Takatsugu Miyazaki, Megumi Ichikawa, Atsushi Nishikawa, Yukari Ohta, Yuko Hidaka, Kinya Katayama, Yuji Hatada, Tetsuya Ito, Koki Fujita (2012) Crystal structure of a lactosucrose-producing enzyme, Arthrobacter sp. K-1 β-fructofuranosidase. Enzyme and Microbial Technology 51, 359–365. https://doi.org/10.1016/j.enzmictec.2012.08.004

4. Mizuki Tamura, Takatsugu Miyazaki, Yutaro Tanaka, Makoto Yoshida, Atsushi Nishikawa, Takashi Tonozuka* (2012) Comparison of the structural changes in two cellobiohydrolases, CcCel6A and CcCel6C, from Coprinopsis cinerea – a tweezer-like motion in the structure of CcCel6C. FEBS Journal 279, 1871–1882. https://doi.org/10.1111/j.1742-4658.2012.08568.x

2011年

3. Takatsugu Miyazaki, Yuji Matsumoto, Kana Matsuda, Yuma Kurakata, Ichiro Matsuo, Yukishige Ito, Atsushi Nishikawa, Takashi Tonozuka* (2011) Heterologous expression and characterization of processing α-glucosidase I from Aspergillus brasiliensis ATCC 9642. Glycoconjugate Journal 28, 563–571. https://doi.org/10.1007/s10719-011-9356-z

2. Kana Matsuda, Yuma Kurakata, Takatsugu Miyazaki, Ichiro Matsuo, Yukishige Ito, Atsushi Nishikawa, Takashi Tonozuka* (2011) Heterologous expression, purification, and characterization of an α-mannosidase belonging to glycoside hydrolase family 99 of Shewanella amazonensis. Bioscience, Biotechnology, and Biochemistry 75, 797–799. https://doi.org/10.1271/bbb.100874

2010年

1. Yuan Liu, Makoto Yoshida, Yuma Kurakata, Takatsugu Miyazaki, Kiyohiko Igarashi, Masahiro Samejima, Kiyoharu Fukuda, Atsushi Nishikawa, Takashi Tonozuka* (2010) Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea. FEBS Journal 277, 1532–1542. https://doi.org/10.1111/j.1742-4658.2010.07582.x

 

総説・記事

4. 宮崎 剛亜* (2020) カイコの糖鎖生物学と糖鎖工学-カイコの糖鎖構造改変と複合型糖鎖生合成酵素の解析、化学と生物 58(6), 330–332. 掲載号表紙

3. 舟根 和美*, 水島 大貴, 宮崎 剛亜, 儀部 茂八, 鏡 朋和, 鈴木 志保, 北村 進一, 原 博 (2017) アンカー型環状イソマルトメガロ糖の生産と用途、New Food Industry 59(5), 1–10.

2. 宮崎 剛亜, 西河 淳, 殿塚 隆史* (2013) GH63 に属するグルコシダーゼの構造と基質特異性に関する新しい知見、応用糖質科学 3(2), 151–158.

1. Takashi Tonozuka*, Takatsugu Miyazaki, Atsushi Nishikawa (2011) Structural similarity between a starch-hydrolyzing enzyme and an N-glycan-hydrolyzing enzyme: exohydrolases cleaving α-1,X-glucosidic linkages to produce β-glucose. Trends in Glycoscience and Glycotechnology 23, 93–102.

著書

1. Takatsugu Miyazaki*, Enoch Y. Park (2019) Chapter 20. Glycobiology and Glycoengineering in Silkworm. Green Science and Technology (Editors: Enoch Y. Park, Takayuki Saito, Hirokazu Kawagishi, Masakazu Hara) CRC Press.